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A comparison of SCCA1 and SCCA2 expression in the peripheral blood mononuclear cells (PBMC)
| Donor III | |||||
|---|---|---|---|---|---|
| Normal PBMC | SCCA1 mRNA | Anti-SCCA1 | SCCA2 mRNA | Anti-SCCA2 | |
| 0 | − | − | − | − | − |
| 1d | + | − | + | − | − |
| 1d/PHA | − | − | − | + | +/− |
| 2d/PHA | − | +/− | − | − | + |
| 3d/PHA | − | − | − | − | − |
| 4d/PHA | − | + | − | − | − |
| 5d/PHA | − | +/− | − | − | + |
| 6d/PHA | − | − | − | − | − |
mRNA expression was evaluated by nested RT-PCR, protein expression was estimated by immunocytochemistry
(+) present, (−) absent, (+/−) trace expression
