Table 2.
Fatty acid composition (percentage of individual lipid class) of Cyclotella cryptica biomass grown under heterotrophic conditions (n = 1)
| Phospholipid | Sterol | Free fatty acid | Triglyceride | |
|---|---|---|---|---|
| 9:0 | n.d. | n.d. | 0.1 | tr. |
| 10:0 | n.d. | n.d. | tr. | tr. |
| 12:0 | tr. | n.d. | 0.1 | tr. |
| 13:0 | 0.1 | 0.3 | n.d. | tr. |
| 14:0 | 1.8 | 1.4 | 1.2 | 2.0 |
| 15:0 | 1.7 | 2.4 | 0.7 | 0.9 |
| 16:0 | 38.2 | 13.0 | 16.5 | 17.9 |
| 17:0 | 0.9 | 0.9 | 0.3 | 0.8 |
| 18:0 | 0.8 | 1.5 | 1.0 | 0.6 |
| 20:0 | tr. | n.d. | n.d. | tr. |
| 22:0 | tr. | n.d. | 0.1 | 0.1 |
| 24:0 | 0.1 | n.d. | 0.2 | 0.1 |
| Sum saturated | 43.6 | 19.4 | 20.3 | 22.3 |
| Trans 16:1 | tr. | n.d. | n.d. | n.d. |
| Trans 18:1 n−9 | tr. | n.d. | n.d. | tr. |
| Trans 18:2 | 0.1 | n.d. | 0.1 | tr. |
| Sum trans | 0.1 | 0.0 | 0.1 | 0.0 |
| 12:1 | tr. | n.d. | n.d. | tr. |
| 13:1 | tr. | 0.1 | n.d. | n.d. |
| 14:1 | n.d. | 0.1 | n.d. | tr. |
| 16:1 n−9 | 0.2 | 0.3 | 0.3 | 0.2 |
| 16:1 n−7 | 26.8 | 45.5 | 31.0 | 57.6 |
| 17:1 | 0.1 | 0.2 | n.d. | 0.3 |
| 18:1 n−9 | 1.6 | 1.6 | 1.7 | 1.1 |
| 18:1 n−7 | 1.1 | 1.0 | 1.1 | 0.8 |
| 19:1 | tr. | n.d. | n.d. | tr. |
| 20:1 n−11 | n.d. | n.d. | n.d. | tr. |
| 22:1 n−11 | 0.6 | 0.5 | 0.5 | 0.3 |
| 22:1 n−9 | n.d. | n.d. | n.d. | tr. |
| 24:1 n−9 | n.d. | n.d. | n.d. | tr. |
| Sum MUFA | 30.5 | 49.3 | 34.5 | 60.3 |
| 18:2 n−9 | 1.1 | 1.0 | 0.4 | 0.6 |
| 20:2 n−9 | tr. | n.d. | n.d. | tr. |
| 20:3 n−9 | tr. | n.d. | n.d. | tr. |
| Sum n−9 | 1.1 | 1.0 | 0.4 | 0.7 |
| 18:2 n−6 | 0.8 | 0.6 | 0.7 | 0.2 |
| 18:3 n−6 | 0.7 | 0.5 | 0.2 | 0.3 |
| 20:3 n−6 | tr. | n.d. | n.d. | tr. |
| 20:4 n−6 | 0.1 | 0.1 | 0.2 | 0.1 |
| 22:2 n−6 | n.d. | n.d. | n.d. | tr. |
| 22:5 n−6 | 1.7 | 3.8 | 2.5 | 0.4 |
| Sum n−6 | 3.3 | 5.1 | 3.7 | 1.0 |
| 16:2 n−3 | 1.8 | 3.3 | 0.2 | 0.4 |
| 18:3 n−3 | 0.1 | 0.2 | n.d. | tr. |
| 18:4 n−3 | 1.9 | 1.4 | 0.5 | 0.9 |
| 20:5 n−3 | 15.8 | 17.8 | 38.0 | 13.9 |
| 22:5 n−3 | tr. | n.d. | n.d. | tr. |
| 22:6 n−3 | 1.8 | 2.7 | 2.2 | 0.5 |
| Sum n−3 | 21.4 | 25.3 | 41.0 | 15.7 |
| Sum PUFA | 25.7 | 31.3 | 45.1 | 17.3 |
Biomass cultivated in a 250-mL Erlenmeyer flask containing 100 mL media and agitated on an orbital mixer at 100 rpm
n.d. not detected, tr. trace, MUFA monounsaturated fatty acid, PUFA polyunsaturated fatty acid