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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4659–4672. doi: 10.4049/jimmunol.1000715

FIGURE 4.

FIGURE 4

Galectin-1 Gal-1hFc induces apoptosis in Th1 and Th17 cell subsets. A, Gal-1hFc binding was assessed on mouse ex vivo polarized Th cell subsets, and Th cell phenotype was assayed by intracellular cytokine staining for IL-4, IFN-γ, and IL-17. dmGal-1hFc and Gal-1hFc binding was performed in the presence or absence of lactose. B, Graphical representations of data from three experiments showing apoptosis (Annexin V+ and PI+) in Th1, Th2, and Th17 cell subsets after a 24-h treatment with 2.5 μM Gal-1hFc in the presence or absence of 50 mM lactose or dmGal-1hFc. *Statistically significant difference compared with hFc control, p ≤ 0.01. C, Representative FACS plots of Gal-1hFc–mediated apoptosis (Annexin V+ and PI+) on Th cell subsets are shown.