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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4659–4672. doi: 10.4049/jimmunol.1000715

FIGURE 9.

FIGURE 9

Galectin-1 human Ig chimera 2 (Gal-1hFc2) modulates cytokine production in T cells draining Ag-sensitized skin and alleviates Ag-dependent inflammation. A, Inguinal LNs draining oxazolone-sensitized skin from mice treated with hFc or Gal-1hFc2 (2.3 mg/kg) were harvested on day 6 after sensitization, minced, and analyzed for cellularity by trypan blue exclusion. Data are expressed as mean 106 cells/LN (±SD). *Statistically significant difference compared with hFc control, p ≤ 0.01. B, Lymphocytes from inguinal LNs were then restimulated for 6 h with PMA/ionomycin/brefeldin A and analyzed for IL-4, IL-10, IL-17, IFN-γ, TGF-β, FoxP3, and CD25 expression by flow cytometry. C, Ears from mice treated with hFc or Gal-1hFc2 were fixed in 10% formalin and stained with H&E. Scale bars, 20 μM. Original magnification ×10; inset, original magnification ×40. D, Change in ear thickness was ascertained 24 h after vehicle alone or oxazolone challenge. All experiments were repeated three times and consisted of three mice per group. *Statistically significant difference compared with hFc-treated mice receiving oxazolone sensitization and challenge, p ≤ 0.01.