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Histopathological observation of mouse spleen at ×100 magnification. (A) Spleen from mice without any toxin injection. (B) Spleen from mice injected with LF mutants and PA shows a normal histology with a clear structure of red and white pulp and normal cell density. (C) Spleen of mice injected with 1× LD50 of LT was seriously damaged, as shown by the disappearance of red and white pulp and a great loss of splenocytes.
