TABLE 1.
Sequence of oligonucleotides used for constructing site-direct mutations in the LF gene
| Mutation | Primer orientationa | Primer sequenceb |
|---|---|---|
| H277A | F | 5′-ATGGGAAAAGATAAAACAGGCCTATCAACACTGG-3′ |
| R | 5′-TAAAGAATCGCTCCAGTGTTGATAGGCCTGTTTTATC-3′ | |
| H280A | F | 5′-AAGATAAAACAGCACTATCAAGCCTGGAGCGATTC-3′ |
| R | 5′-TTCAGATAAAGAATCGCTCCAGGCTTGATAGTGC-3′ | |
| H309A | F | 5′-AGAAAGATGACATAATTGCTTCTTTATCTCAAGAAGA-3′ |
| R | 5′-TCTTCTTGAGATAAAGAAGCAATTATGTCATCTTTCT-3′ | |
| H424A | F | 5′-ATTGATGCTTTATTAGCTCAATCCATTGGAAGTACCTTG −3′ |
| R | 5′-CAAGGTACTTCCAATGGATTGAGCTAATAA AGCATCAAT-3′ | |
| H588A | F | 5′-GCTTATTACATTCAACGTGGCTAATAGATATGCATCC-3′ |
| R | 5′-GGATGCATATCTATTAGCCACGTTGAATGTAATAAGC-3′ | |
| H645A | F | 5′-GCTGAACAATATACAGCTCAAGATGAGATATATGAGC-3′ |
| R | 5′-GCTCATATATCTCATCTTGAGCTGTATATTGTTCAGC-3′ | |
| H654A | F | 5′-GATGAGATATATGAGCAAGTTGCTTCAAAAGGGT-3′ |
| R | 5′-CATATAACCCTTTTGAAGCAACTTGCTCATATATCTC-3′ | |
| H669A | F | 5′-GAATCCCGTTCTATATTACTCGCTGGACCTTCA-3′ |
| R | 5′-CTACA CCTTTTGAAGGTCCAGCGAGTAATATAG-3′ | |
| H669R | F | 5′-GAATCCCGTTCTATATTACTCAGAGGACCTTCA-3′ |
| R | 5′-CTACA CCTTTTGAAGGTCCTCTGAGTAATATAG-3′ | |
| H745A | F | 5′-GCAGAAGCCT TTAGGTTAATGGCTTCTACGGACCAT-3′ |
| R | 5′-AACGTTCAGCATGGTCCGTAGAAGCCATTAACCTAA-3′ | |
| H749A | F | 5′-AGGTTAATGCATTCTACGGACGCTGCTG AACGTTTA-3′ |
| R | 5′-TTGAACTTTTAAACGTTCAG CAGCGTCCGTAGAATGC-3′ |
a
F, forward; R, reverse.
b
Mutated residues are shown in boldface.