TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype or descriptiona | Construction or reference |
|---|---|---|
| 168 | trpC2 | Lab strain |
| CU1065 | trpC2attSPβ | Lab strain |
| JH642 | trpC2 pheA1 | 5 |
| NCIB 3610 | Undomesticated wild strain | Lab strain |
| HB10107 | 168 sigM::tet sigX::kan sigW::mls | 34 |
| BSU2007 | 168 ΔsigMΔsigXΔsigWΔsigYΔsigVΔsigZΔylaC | 2 |
| HB0009 | CU1065 sigY::mls | 16 |
| HB0911 | CU1065 sigV::cat | 35 |
| HB0915 | CU1065 ylaC::spec | 35 |
| HB0032 | CU1065 sigZ::kan | 15 |
| HB10101 | 168 sigV::cat | ChrDNA HB0911→168 |
| HB10108 | 168 sigY::mls | ChrDNA HB0009→168 |
| HB10109 | 168 sigZ::kan | ChrDNA HB0032→168 |
| HB10233 | 168 ylaC::spec | ChrDNA HB0915→168 |
| HB10234 | 168 sigY::mls ylaC::spec | ChrDNA HB10108→HB10233 |
| HB10235 | 168 sigZ::kan sigV::cat | ChrDNA HB10109→HB10101 |
| HB10236 | 168 sigV::cat sigY::mls sigZ::kan ylaC::spec | ChrDNA HB10234→HB10235 |
| YC125 | NCIB3610 ΔepsH::tetamyE::PlutA-lacZ (Cmr) | 18 |
| HB10223 | 168 epsH::tet | ChrDNA YC125→168 |
| HB10158 | 168 amyE::Pspac-abh (Cmr) | ChrDNA BZH73→168 |
| HB10224 | 168 ΔsigMΔsigXΔsigWΔsigYΔsigVΔsigZΔylaCamyE::Pspac-abh (Cmr) | ChrDNA HB10158→ BSU2007 |
| HB10225 | 168 sigM::tet sigX::kan sigW::mls amyE::Pspac-abh (Cmr) | ChrDNA HB10158→ HB10107 |
| JMA208 | JH642 immR::cat | 5 |
| JMA222 | JH642 ICEBs10/cured of ICEBs1 | 5 |
| BZH73 | JH642 abh::kan amyE::Pspac-abh (Cmr) thrC::PsunA-lacZ (Specr) | 44 |
a
Cmr, chloramphenicol resistance; Specr, spectinomycin resistance.