FIG. 3.

Transcription from MotA-dependent T4 middle promoters is specifically reduced in the rpoB(E835K G1249D) mutant strain B11 versus the wt rpoB strain BL21(DE3). Histograms show the levels of RNA from various middle promoters (top two panels) or early promoters (bottom panel) made in the indicated infections: solid black, wt T4/BL21(DE3); solid red, wt T4/B11; open black, T4 motA(Am)/BL21(DE3); open red, T4 motA(Am)/B11. A portion of the gel displaying the indicated primer extension products for each promoter is also shown. [Values for T4 motA(Am) infections were obtained using longer exposures.] For cases in which the primer extension analyses were performed three or more times, an error bar is shown. In the other cases, values from two analyses were averaged. Pm_uvsX RNA has multiple ends due to the iterative addition of As at the start of the transcript (15).