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The rpoB mutation G1249D is responsible for the defect in MotA/AsiA-activated transcription in a wt T4 infection. Portions of gels showing primer extension products were obtained after wt T4 infections of the following: wt rpoB strain BL21(DE3) (lane 1), the double mutant rpoB(E835K G1249D) strain B11 (lane 2), B11 containing pE835K/wt1249 (lane 3), and B11 containing pwt835/G1249D.
