TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description and relevant characteristicsa | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAK | P. aeruginosa strain K, wild type | 54 |
| PAK::PexoS-lacZ | PAK with chromosomal exoS promoter reporter | 12 |
| PAKmucA22::PexoS-lacZ | mucA22 with chromosomal exoS promoter reporter | This study |
| PAKΔalgD::PexoS-lacZ | algD with chromosomal exoS promoter reporter | This study |
| PAKΔalgDmucA22::PexoS-lacZ | algD mucA22 with chromosomal exoS promoter reporter | This study |
| PAKΔcyaAB::PexoS-lacZ | cyaAB with chromosomal exoS promoter reporter | 12 |
| PAKΔvfr::PexoS-lacZ | vfr with chromosomal exoS promoter reporter | This study |
| PAK::lacP1ΔlacI-lacZ | PAK with chromosomal CVS reporter | 13 |
| PAKmucA22::lacP1ΔlacI-lacZ | mucA22 with chromosomal CVS reporter | This study |
| PAKΔmucA::lacP1ΔlacI-lacZ | mucA with chromosomal CVS reporter | This study |
| PAKΔalgD::lacP1ΔlacI-lacZ | algD with chromosomal CVS reporter | This study |
| PAKΔalgDmucA22::lacP1ΔlacI-lacZ | algD mucA22 with chromosomal CVS reporter | This study |
| PAKΔcyaAB::lacP1ΔlacI-lacZ | cyaAB with chromosomal CVS reporter | 13 |
| PAKΔvfr::lacP1ΔlacI-lacZ | vfr with chromosomal CVS reporter | 13 |
| PAKΔalgZR::lacP1ΔlacI-lacZ | algZR with chromosomal CVS reporter | This study |
| PAKΔcyaABmucA22::lacP1ΔlacI-lacZ | cyaAB mucA22 with chromosomal CVS reporter | This study |
| PAKΔvfrmucA22::lacP1ΔlacI-lacZ | vfr mucA22 with chromosomal CVS reporter | This study |
| PAKΔalgU::lacP1ΔlacI-lacZ | algU with chromosomal CVS reporter | This study |
| PAKΔalgUmucA22::lacP1ΔlacI-lacZ | algU mucA22 with chromosomal CVS reporter | This study |
| PAKΔalgZR::lacP1ΔlacI-lacZ | algZR with chromosomal CVS reporter | This study |
| PAKΔalgZRmucA22::lacP1ΔlacI-lacZ | algZR mucA22 with chromosomal CVS reporter | This study |
| PAK::Pvfr-lacZ | PAK with chromosomal vfr promoter reporter | 12 |
| PAKΔvfr::Pvfr-lacZ | vfr with chromosomal vfr promoter reporter | 12 |
| PAKmucA22::Pvfr-lacZ | mucA22 with chromosomal vfr promoter reporter | This study |
| FRD1 | Mucoid CF clinical isolate with mucA22 allele | 42 |
| PAO1 | P. aeruginosa laboratory-passaged strain, wild type | 23 |
| PAO1mucA22 | mucA22 mutation in PAO1 | This study |
| PA103 | P. aeruginosa laboratory-passaged strain, wild type | 29 |
| PA103mucA22 | mucA22 mutation in PA103 | This study |
| E2 | P. aeruginosa environmental isolate, wild type | 58 |
| E2mucA22 | mucA22 mutation in E2 | This study |
| MSH3 | P. aeruginosa environmental isolate, wild type | 58 |
| MSH3mucA22 | mucA22 mutation in MSH3 | This study |
| Plasmids | ||
| pDONR201 | Gateway cloning vector; Kmr | Invitrogen |
| pEX18Gm | Suicide vector; Gmr | 21 |
| pDONRX | Gateway-adapted suicide vector; Kmr | 13 |
| pDONRXmucA22 | Suicide vector containing mucA22 allele; Gmr | This study |
| pDONRXΔmucA | Suicide vector containing mucA deletion allele; Gmr | This study |
| pDONRXΔalgU | Suicide vector containing algU deletion allele; Gmr | This study |
| pEXGmΔalgD | Suicide vector containing algD deletion allele; Gmr | This study |
| pEXGmΔalgZR | Suicide vector containing algZR deletion allele; Gmr | This study |
| pEXGmΔvfr | Suicide vector containing vfr deletion allele; Gmr | 59 |
| pEXGmΔcyaA | Suicide vector containing cyaA deletion allele; Gmr | 59 |
| pEXGmΔcyaB | Suicide vector containing cyaB deletion allele; Gmr | 59 |
| pMMB67EH | Empty P. aeruginosa expression vector; Apr | 14 |
| pMMBV1GW | Gateway-adapted version of pMMB67EH; Apr | 13 |
| pPa-cyaB | P. aeruginosa cyaB carried on pMMBV2; Apr | 13 |
| pPa-vfr | P. aeruginosa vfr carried on pMMBV1; Apr | 12 |
| pPa-algZR | P. aeruginosa algZR carried on pMMBV1; Apr | This study |
| pPa-mucA | P. aeruginosa mucA carried on pMMBV1; Apr | This study |
| pUS150 | Plasmid containing wild-type algR; Apr | 31 |
| pJN105 | Arabinose-inducible expression plasmid; Gmr | 41 |
| pJN-algR | P. aeruginosa algR carried on pJN105; Gmr | This study |
| pJN-algZ | P. aeruginosa algZ carried on pJN105; Gmr | This study |
| pJN-algZR | P. aeruginosa algZR carried on pJN105; Gmr | This study |
a
Apr, ampicillin resistance marker; Kmr, kanamycin resistance marker; Gmr, gentamicin resistance marker.