TABLE 2.
Mass spectrometry analysis of Vi phage I
| Gene designationa | Description | Mol wt | No. of peptide sequences identified per protein |
|---|---|---|---|
| Vi01_006 | Putative tail protein product 5503:6312 | 27,801 | 13 |
| Vi01_034 | T4-like baseplate tail tube cap 20271:21239 | 36,118 | 8 |
| Vi01_035 | Gp53 baseplate wedge subunit 21252:21806 | 21,534 | 8 |
| Vi01_036 | Conserved phage associated protein 21806:23194 | 52,590 | 16 |
| Vi01_037 | Phage-associated protein 23205:25145 | 70,019 | 22 |
| Vi01_065c | Gp2 DNA end protector protein 41170:41874 rev | 28,329 | 11 |
| Vi01_066 | Baseplate tail tube initiator 41929:42873 | 35,020 | 5 |
| Vi01_076 | Baseplate hub subunit 48036:49661 | 59,079 | 7 |
| Vi01_077 | Gp25 baseplate wedge protein 49726:50106 | 14,119 | 7 |
| Vi01_081c | Glutaredoxin 51249:51476 rev | 8,472 | 1 |
| Vi01_092c | Conserved phage-associated protein 60113:60466 rev | 13,026 | 3 |
| Vi01_093c | Phage-associated protein 60527:61099 rev | 20,383 | 7 |
| Vi01_094c | Phage-associated protein 61147:63546 rev | 87,994 | 108 |
| Vi01_096c | Phage-associated protein 64004:64642 rev | 22,864 | 4 |
| Vi01_105 | Conserved phage-associated protein 71048:71815 | 28,321 | 15 |
| Vi01_112c | Phage-associated protein 75633:75920 rev | 10,868 | 1 |
| Vi01_117c | Phage-associated protein 78753:79199 rev | 17,304 | 15 |
| Vi01_140c | Tail completion and sheath stabilizer protein 95723:96217 rev | 18,533 | 2 |
| Vi01_142c | Conserved phage-associated protein 96906:97631 rev | 25,368 | 3 |
| Vi01_149c | Phage-associated protein 99867:100310 rev | 16,901 | 2 |
| Vi01_152c | Major capsid protein 100868:102190 rev | 47,971 | 36 |
| Vi01_154c | Gp21 prohead protease 103168:103836 rev | 24,454 | 10 |
| Vi01_155c | Conserved phage-associated protein 103847:104161 rev | 12,349 | 5 |
| Vi01_157c | Gp20 portal vertex protein of the head 104382:106064 rev | 63,067 | 48 |
| Vi01_158c | Gp19 tail tube protein 106135:106665 rev | 19,900 | 10 |
| Vi01_160c | Gp18 tail sheath protein 107211:109106 rev | 68,380 | 32 |
| Vi01_163c | Gp15 proximal tail sheath stabilization 112033:112731 rev | 26,876 | 2 |
| Vi01_164c | Gp14 neck protein 112734:113375 rev | 24,743 | 6 |
| Vi01_166c | Gp13 neck protein 113678:114430 rev | 29,000 | 11 |
| Vi01_169c | Conserved phage-associated protein 115043:119881 rev | 177,452 | 87 |
| Vi01_170c | Tail spike protein 119976:121766 rev | 64,411 | 25 |
| Vi01_171c | Maturation-adhesion tail spike protein 121816:124014 rev | 79,294 | 31 |
| Vi01_172c | Tail spike protein 124070:126610 rev | 91,415 | 26 |
| Vi01_173c | Hemolysin-type calcium-binding protein 126661:129834 rev | 111,484 | 41 |
| Vi01_174c | Putative tail fiber protein 129881:131071 rev | 42,525 | 35 |
| Vi01_175c | Conserved phage-associated protein 131074:131928 rev | 33,009 | 7 |
| Vi01_176c | Gp6 baseplate wedge subunit 131912:133693 rev | 65,582 | 35 |
| Vi01_179 | Phage-associated protein (Vi phage I only) 138943:139176 | 8,054 | 3 |
| Vi01_180 | Conserved phage-associated protein 139186:139701 | 18,551 | 4 |
| Vi01_183 | Phage-associated protein (Vi phage I only) 141852:142301 | 16,820 | 7 |
| Vi01_184 | Phage-associated protein 142337:142792 | 17,332 | 1 |
a
A significant number of CDSs were identified during this analysis, including those for many hypothetical proteins and a full range of structural proteins. These included the major capsid, collar, baseplate, and all three predicted tail spike proteins.