TABLE 3.
Mass spectrometry analysis of Vi phage VIa
Gene designation | Description | Equivalent gene in phage T7 | Mol wt | No. of peptide sequences identified per protein |
---|---|---|---|---|
Vi06_30 | Conserved hypothetical phage protein 16148:16414 | gp6.7 | 9,450 | 13 |
Vi06_32 | Host specificity protein B 16819:17092 | gp7.3 | 9,862 | 13 |
Vi06_33 | Predicted head-to-tail joining protein 17107:18717 | gp8 | 59,029 | 47 |
Vi06_34 | Predicted capsid assembly protein 18760:19692 | gp9 | 34,075 | 7 |
Vi06_35 | Predicted major capsid protein 19829:20881 | gp10A | 36,993 | 42 |
Vi06_37 | Predicted tail tubular protein A 21082:21672 | gp11 | 22,278 | 14 |
Vi06_38 | Predicted tail tubular protein B 21693:24080 | gp12 | 89,481 | 47 |
Vi06_40 | Predicted internal virion protein B 24589:25179 | gp14 | 20,778 | 26 |
Vi06_41 | Predicted internal virion protein C 25186:27429 | gp15 | 84,421 | 93 |
Vi06_42 | Predicted internal virion protein D 27456:31412 | gp16 | 143,700 | 105 |
Vi06_43 | Maturation-adhesion tail fiber protein 31494: 33467 | gp17 | 71,792 | 43 |
This phage represents the array of Podoviridae phages that are the predominant type found in this Vi phage collection. A array of CDSs similar to those found with mass spectrometry of T7 phage were identified.