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Dependence on protein for excision of uracil from DNA by A. fulgidus cell extract (A) and rAfung (B). Different amounts of cell extract (A) or rAfung (B) were incubated with [3H]uracil-containing DNA (2,400 dpm; 2.2 pmol DNA uracil residues) in reaction buffer at 80°C for 10 min. Each value is the average of two independent measurements.
