FIG. 3.

Western blot analysis of the Afung protein present in A. fulgidus cell extract. Following SDS-PAGE of A. fulgidus cell extract and purified rAfung and rAfung^H (the results of one such experiment performed with a 12% [wt/vol] polyacrylamide gel stained with Coomassie blue are shown in panel A), Western blot analysis (B) using antiserum raised against rAfung^H (left panel, diluted 1/4,000; right panel, diluted 1/1,000) was performed with purified rAfung and rAfung^H and cell extract as described in Materials and Methods.