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Lineweaver-Burk plots for excision of uracil from DNA by wtAfung (A) and rAfung (B). Protein extract (14 μg containing 0.33 pmol wtAfung) or rAfung (6.2 pmol) was incubated with different amounts of [3H]uracil-containing DNA (7.55 to 75.5 pmol DNA uracil residues) in reaction buffer at pH 7.5 for 10 min at 80°C. Each value is the average of two to six independent measurements.
