TABLE 2.
Characterized UDGs of organisms that thrive at high temperaturesa
| Organism | Protein | ORF | Family | Mr | No. of amino acids | UDG activityb | Reference(s) |
|---|---|---|---|---|---|---|---|
| Euryarchaeota | |||||||
| Archaeoglobus fulgidus | Afungc | AF2277 | 4 | 22,718 | 199 | + (ssDNA TC > G > A) | 31 |
| Methanobacterium thermoautotrophicum | MthMIG | pFV1-Orf10 | MIG | 25,386 | 221 | + (G > C) − (ssDNA) | 23 |
| Methanocaldococcus jannaschii | MjUDG | MJ1434 | 6 | 25,938 | 220 | + (TCGA) | 7 |
| Pyrococcus furiosus | PfuUDG | PF1385 | 4 | 22,335 | 196 | + (ssDNA GA) | 28 |
| Crenarchaeota | |||||||
| Pyrobaculum aerophilum | PaUDGac | PAE0651 | 4 | 21,563 | 196 | + (G > A) | 49, 51 |
| PaUDGb | PAE1327 | 5 | 24,914 | 226 | + (G > A > ssDNA) | 49 | |
| PaMIG | PAE3199 | MIG | 26,446 | 230 | + (G) − (ATC) | 70 | |
| Sulfolobus solfataricus | UDG1 | SSO2275 | 4 | 24,367 | 216 | + (G > ssDNA > A) | 11 |
| Bacteria | |||||||
| Thermotoga maritima | TmUDG | TM0511 | 4 | 21,501 | 192 | + (GA) | 47 |
| Thermus thermophilus | TtUDGa | TTHA0718 | 4 | 22,966 | 205 | + (C > G > A, ssDNA) | 59 |
| TtUDGb | TTHA1149 | 5 | 24,286 | 219 | + (G > C > A) − (ssDNA) | 59 |
a
Families 1, 2, and 3 have not been identified in hyperthermophiles.
b
The opposite bases are indicated in parentheses.
c
The protein interacts with PCNA (proliferating cell nuclear antigen; sliding clamp) homologues, as demonstrated for PaUDGa or suggested for Afung because of a putative PCNA-binding motif (69).