FIG. 3.

Average profiles of transcriptional and σ^A, β′, and NusA binding signals on 180 high-quality TUs. Relative signal intensities of the transcripts (A) and σ^A (B), β′ (C), and NusA (D) binding to those at TSSs (y axis) are plotted as a function of distance from TSSs (x = 0) and connected by red lines for selected 180 TUs. The average profiles are calculated using the LOWESS smoothing method and are presented as green lines (A to D). (E) Average profiles are merged after adjusting values at the TSSs to a log₁₀ of 1. Solid and dashed lines indicate average profiles of data sets presented in this figure and in Fig. S2 in the supplemental material, respectively. (F) Box-and-whisker plots showing the distributions of peak positions of σ^A, β′, and NusA binding, determined by using duplicated data. The upper and lower limits of the boxes indicate the 75th and 25th percentiles, respectively, and the boldface lines across the boxes indicate median values. Outliers with values more than 1.5 times the interquartile range of the 75th percentile or less than 1.5 times the interquartile range of the 25th percentile are shown as open circles.