TABLE 2.
Bacterial strains and vectors used in this study
| Strain or vector | Characteristic(s) | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli DH5α | Cloning host | TaKaRa |
| M. smegmatis mc2155 | Expression host | 29 |
| M. intracellulare ATCC 35767 | MAC serovar 4 strain | 35 |
| M. avium JATA51-01 | Source of the rtfA gene | 26 |
| Vectors | ||
| pYM301a | Site-specific integrating mycobacterial vector carrying an hsp60 promoter cassette | 25 |
| pMV261a | E. coli-Mycobacterium shuttle vector carrying an hsp60 promoter cassette with an AflII site | This study |
| pMVΔmtfF | Source of mdhtA, merA, and gtfD genes | 26 |
| pMV-rtfA-mdhtA-merA-gtfD | pMV261a carrying rtfA, mdhtA, merA, and gtfD genes | This study |
| pYM-hlpA | pYM301a carrying the hlpA gene | This study |
| pYM-hlpA-orf2 | pYM301a carrying the hlpA gene and ORF2 | This study |
| pYM-orf3-orf4-orf5 | pYM301a carrying ORF3, ORF4, and ORF5 | This study |