TABLE 3.

Oligonucleotide primers used in this study

Primer	Sequencea	Restriction site
RTFA-S	5′-CGGGATCCCATGAAATTTGCTGTGGCAAG-3′	BamHI
RTFA-A	5′-AACTGCAGCTCAGCGACTTCGCTGCGCTTC-3′	PstI
MDHTA-S2	5′-GCTCTAGACTGCAGAAAAACCAACTTCTACTGCCTGACCTG-3′	PstI
GTFD-A2	5′-GGAATTCTTAAGTCTACGGTTCTGCGCTTCGTTCTTTG-3′	AflII
HLPA-S	5′-GGAATTCGTGACAACGACGCCACCAGT-3′	EcoRI
HLPA-A	5′-CCATCGATACTACGCTGCCGCGCTAGGCG-3′	ClaI
ORF2-A	5′-CCCAAGCTTCTCAGACTCTAACGTACAGTTC-3′	HindIII
ORF3-S	5′-CACCTGCAGAAATGACCGCCACAACCAGGGC-3′	PstI
ORF5-A	5′-GCAGAATTCCTACGGCGCCAATTCGATGAG-3′	EcoRI
GTFB-S1	5′-GGAACTCCTGCACCTTGGGGCCGT-3′
MDHTA-A2	5′-GGTGCGGGTCAACGTAGAGGTG-3′

^aUnderlining indicates the restriction site.