TABLE 5.

Enhanced expression of spoIIGA in the prespore results in σ^E activity in the prespore

a σ^E activity was visualized using a P_spoIID-gfp fusion. Strains contained the csfB::cat mutation. Cultures were induced to form spores in MSSM. Samples were analyzed 2.5h after the start of spore formation. Schematic representations are given of the different types of organism present in the cultures; shaded areas indicate GFP fluorescence. Membranes were visualized by staining with FM4-64. For clarity, data for SL14715 (2h) have been recapitulated from Table 3.