Reversed-phase HPLC of the PQQ. (A) Reversed-phase HPLC chromatogram of the methanol extracted quinones associated with the purified acetaldehyde dehydrogenase complex (3.0 mg of protein) of Ga. diazotrophicus. (B) The system was calibrated with commercial standards: PQQH2 (commercial form) and commercial PQQH2 oxidized with ammonium persulfate, Q7, and Q10 (retention times of 4.28, 6.8, 9.28, and 11.19 min, respectively).