FIG. 7.

Oxidation of cytochromes c and b associated with the aldehyde dehydrogenase complex by the electron acceptor UQ₁. Purified ALDH (46 μg of protein) was stepwise reduced by limited amounts of dithionite, and the full reduced spectrum was traced. At this point, the oxidation of the ALDH complex was induced by successive additions of UQ₁ (concentrations are noted in the inset). The oxidation of cytochromes c (ΔA_550-540) and b (ΔA_556-570) was measured and plotted against the UQ₁ concentration reached after each addition (inset).