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Purification summary of the membrane-bound ALDH
| Purification step | Total protein (mg) | Sp act (U/mg)a | Total activity (U) | Yield (%) |
|---|---|---|---|---|
| Membrane fraction | 3,000 | 5 | 15,000 | 100 |
| Solubilized fraction | 600 | 18 | 10,800 | 72 |
| QAE-Toyopearl | 250 | 32 | 8,000 | 53 |
| Ha-Ultrogel | 15 | 393 | 5,895 | 39 |
| Sephacryl S200 | 4 | 947 | 3,788 | 25 |
The activity was assayed under the standard conditions described in Materials and Methods.
