FIG. 7.

Subcellular localization of AT C-terminal domains with a deleted or heterologous α-helix. Western blots were probed with anti-E tag MAb to detect Jun-AT constructs in the indicated subcellular fractions obtained from induced E. coli cells: soluble, envelope, TX-100-solubilized, urea-solubilized, and integral OMPs (see Materials and Methods). The detection of the periplasmic MBP and OmpA with specific antibodies (indicated in the panels) was done to control the E. coli fractionation method. The masses of protein standards are shown on the left (in kDa).