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Pull-down assay of extract from S. coelicolor M145 grown for 3 days on solid MM. Samples were analyzed by 17.5% SDS-PAGE. Lane S-3d, eluted sample of proteins from S. coelicolor M145 extracts bound on the UshX column as described in Materials and Methods; lanes UshX and σH37, purified His-tagged proteins after Ni2+ affinity chromatography; lane M, molecular mass markers. The position of the 12.5-kDa protein band used for the tandem mass spectrometry is indicated by an arrow.
