FIG. 7.

FimZ controls the dynamics of SPI1 gene expression. (A to C) Deleting FimZ increases the duration of SPI1 gene expression. P_flgA (A), P_hilA (B), and P_fimA (C) promoter activities in the wild type and the ΔfimZ mutant are shown. (D to F) Overexpressing FimZ represses both flagellar and SPI1 gene expression. P_flgA (D), P_hilA (E), and P_fimA (F) promoter activities in the wild type and the P_fimZ::tetRA mutant, where FimZ is under the control of a tetracycline-inducible promoter, are shown. FimZ expression was induced by addition of 15 μg/ml tetracycline. Experiments were performed as described for Fig. 2.