Table 1.
Oligonucleotides used in the study[a]
| Name | Sequence | Purification |
|---|---|---|
| UMB | FAM-5′-CGC GTT AAC ATA CAA TAG ATC GCG-BHQ1 |
double HPLC |
| UMB′ | FAM-5′-CGC GTT AAC ATA CAA TAG ATC GCG |
HPLC |
| C18 | 5′-GAT CTA TTG TAT GTT AAC | SD[b] |
| C22 | 5′-GC GAT CTA TTG TAT GTT AAC GC | SD |
| C24 | 5′-CGC GAT CTA TTG TAT GTT AAC GCG | SD |
| BDP rs14-f | 5′-CCT GCT CTG AGG CCA GC/TEG/TAT GTT AAC |
SD |
| BDP rs14m-A | 5′-GAT CTA TTG/TEG/CAG TTT TGC | SD |
| BDP rs14m-G | 5′-GAT CTA TTG/TEG/CAG TTC TGC | SD |
| BDP rs71-f | 5′-ACC TAA TGA CAG ACG T/TEG/TAT GTT AAC |
SD |
| BDP rs71m-A | 5′-GAT CTA TTG/TEG/TCA CTA CAC | SD |
| BDP rs71m-G | 5′-GAT CTA TTG/TEG/TCA CCA CAC | SD |
| BDP rs87-f | 5′-TA GCA GAG TGT GAC AAA/TEG/TAT GTT AAC |
SD |
| BDP rs87m-C | 5′-GAT CTA TTG/TEG/AAA TGT ACT | SD |
| BDP rs87m-T | 5′-GAT CTA TTG/TEG/AAA TAT ACT | SD |
| rs14-G | 5′-ACT GGG CTG ATG TGG GTT CTT TGC AGA ACT G GC TGG CCT CAG AG C AGG GA |
SD |
| rs14-A | 5′-ACT GGG CTG ATG TGG GTT CTT TGC AAA ACT G GC TGG CCT CAG AG C AGG GA |
SD |
| rs71-A | 5′-GAA AGG CAT ATC GTA TTA ACT GTG TAG TGA ACG TCT GTC ATT AGG TTT AGC |
SD |
| rs71-G | 5′-GAA AGG CAT ATC GTA TTA ACT GTG TGG TGA ACG TCT GTC ATT AGG TTT AGC |
SD |
| rs87-C | 5′-ATA CCA CTG CAC TGA AGT ATA AGT ACA TTT TTT GTC ACA CTC TGC TA A CT |
SD |
| rs87-T | 5′-ATA CCA CTG CAC TGA AGT ATA AGT ATA TTT TTT GTC ACA CTC TGC TAA CT |
SD |
Stem-forming nucleotides of MB probe are shown in italics, “/TEG/” indicates triethylene glycol linker inserts; the underlined nucleotides indicate the SNP sites and the positions complementary to SNPs.
SD is standard desalting.