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. Author manuscript; available in PMC: 2011 Oct 8.
Published in final edited form as: Mol Cell. 2010 Oct 8;40(1):112–125. doi: 10.1016/j.molcel.2010.09.007

Figure 3. The MRS and the histone variant H2A.Z control peripheral targeting of recently repressed INO1.

Figure 3

A. Scheme for integrating DNA elements for localization experiments (Ahmed et al., 2010). B. Peripheral localization of URA3, URA3:MRS and URA3:MRS in the htz1Δ strain. C. Chromatin immunoprecipitation of HA-H2A.Z (Meneghini et al., 2003) from wild type and mrs mutant INO1strains and quantified using primers to amplify −197 to −284 relative to the INO1 ORF or primers to amplify the BUD3 promoter. D. Top panel: map of nucleosomes in the INO1 promoter. Shown are the positions of GRS I (red box), the MRS (yellow box), the TATA box (grey box), two UASINO elements (green boxes) and the PCR products associated with each nucleosome. Bottom panel: ChIP of HA-H2A.Z from either a wild type or mrs mutant INO1 strain, quantified using primers corresponding to the locations in the top panel or the BUD3 promoter.