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. Author manuscript; available in PMC: 2011 Oct 8.
Published in final edited form as: Mol Cell. 2010 Oct 8;40(1):112–125. doi: 10.1016/j.molcel.2010.09.007

Figure 5. The MRS is required for transcriptional memory.

Figure 5

A. INO1 activation. At time = 0, cells were shifted from repressing medium containing 100μM inositol (red arrow in schematic) to medium without inositol (green arrow in schematic). Cells were harvested at indicated time points and INO1 mRNA levels were quantified relative to ACT1 mRNA levels by RT-qPCR. B. INO1 reactivation. Cells were shifted from activating medium to repressing medium containing 100μM inositol for 3h. At time = 0, cells were harvested and returned to medium without inositol. Cells were harvested at indicated time points and INO1 mRNA levels were quantified relative to ACT1 mRNA levels by RT-qPCR. C. ChIP with anti-Rbp1 antibody at the indicated time points during activation (same scheme as in A). D. ChIP with anti-Rbp1 antibody at the indicated time points during reactivation (same scheme as in B). E. ChIP with anti-Rbp1 antibody (8WG16) from wild type or mrs mut INO1 strains after repression. F. ChIP using anti-Rpb1, anti-phospho Ser2 CTD or anti-phospho Ser5 CTD antibodies from wild type or mrs mut INO1 strains under activating conditions or after 3h of repression.