Fig. 3.

Analysis of reduced folate carrier (RFC) and dehydrofolate reductase (DHFR) protein levels. The protein expression level of RFC and DHFR in AGS and Saos-2 cell lines. AGS and Saos-2 cells treated with methotrexate (MTX) at 0, 1, 10, 100 and 1,000 nM for 72 hr. Equal amounts of total cell lysates were used for loading and detected by Western blotting. The experiments were repeated at least three times independently. β-actin was used as control.