Skip to main content
. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Nature. 2010 Aug 18;467(7314):430–435. doi: 10.1038/nature09380

Figure 1. Mediator and Cohesin Contribute to ES Cell State.

Figure 1

a, Mediator and Cohesin components were highly represented in an shRNA screen for regulators of ES cell state. Complete results are listed in Supplementary Tables 1, 2. b, Knockdown of Mediator (Med12), Cohesin (Smc1a) or Nipbl caused reduced Oct4 protein levels and changes in ES cell colony morphology. Murine ES cells were infected with GFP control, Med12, Smc1a or Nipbl shRNAs, and stained for Oct4 and with Hoechst. Scale bar = 100μM. c, Mediator, Cohesin and Nipbl knockdowns all cause reduced expression of ES cell regulators and increased expression of developmental regulators. ES cells were infected with the indicated shRNA and gene expression levels relative to a control GFP infection were determined with microarrays. Log2 fold expression changes were rank ordered from lowest to highest for all genes.