Table 3.
Intrinsic metabolic activities of DMSO-treated Huh7 cells.
| Differentiated Huh7 | Human hepatocytes | References | |
|---|---|---|---|
| Midazolam | |||
| 1-Hydroxymidazolam production rate (pmol min−1 per 106 cells) | 0.87 | 10–28 | Kanebratt and Andersson (2008), Ledirac et al. (2000), |
| Intrinsic clearance (μl min−1 per 106 cells) | 0.00059 | 0.0015–0.04 | Hallifax et al. (2005), Li et al. (1997) |
| Lamotrigine | |||
| Intrinsic clearance (μl min−1 per 106 cells) | 0.00099 | 0.11 (liver microsome)a | Rowland et al. (2006) |
| Diclofenac | |||
| 4-Hydroxydiclofenac production rate (pmol min−1 per 106 cells) | 0.189 | 300 | Hewes et al. (2006) |
Based on previous reports indicating that 1 g of liver tissue is equivalent to 40 mg microsomal proteins (Hakooz et al. 2006) and 108 hepatocytes (Wilson et al. 2003).