Table 2.
Limits-of-detection (LODs) for bacterial and toxin antigens in PBST and complex food matrices using MagPlex microspheres
| PBST | Alfalfa | Lettuce | Spinach | Tomato | Pepper | Apple juice | Milk | Chicken wash | Ground beef | |
|---|---|---|---|---|---|---|---|---|---|---|
| E. coli (cfu/mL) | 103 | 105 | 105 | 105 | 104 | 103 | 105 | 105 | 104 | 105 |
| Salmonella (cfu/mL) | 106 | 106 | 104 | 106 | 107 | 107 | 106 | 107 | 106 | 106 |
| Campylobacter (cfu/mL) | 103 | >107a | >107 | 106 | >107 | 107 | 107 | 107 | 106 | 107 |
| Listeria (cfu/mL) | 102 | 107 | >107 | 106 | 104 | 105 | 104 | 106 | 106 | 104 |
| SEB (ng/mL) | 0.064 | 0.32 | 8.0 | 0.32 | 0.064 | 1.6 | 0.32 | 1.6 | 1.6 | 0.32 |
Bacteria LODs are measured in colony forming units per milliliter and toxin LODs in nanograms in milliliters. LODs were determined as the concentration actually measured that has a mean fluorescence signal greater the mean background fluorescence signal plus three times the standard deviation
Not detectable within the concentration range of 101–107 cfu/mL