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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Cell Commun Insights. 2009;2:1–11. doi: 10.4137/cci.s2775

Figure 3.

Figure 3

Function-blocking PAI-1 antibodies disrupt Matrigel-induced tubular network formation in human microvessel endothelial cells. HMEC-1 cells formed well-developed capillary-like structures with patent lumens within 24 hours after plating onto hydrated Matrigel coatings. (A) Network maintenance required fine regulation of the plasmin-generating cascade as addition of plasminogen (+plasminogen) (B) or PAI-1 neutralizing antibodies (+neutralizing IgG) (C) separately effectively inhibited tubular differentiation and promoted network breakdown consistent with the requirement of PAI-1 for capillary stability.15 The simultaneous addition of plasminogen and PAI-1 IgG (D) resulted in complete network disassembly with reversion to a more monolayer growth pattern.