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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Cell Commun Insights. 2009;2:1–11. doi: 10.4137/cci.s2775

Figure 4.

Figure 4

PAI-1 rescues T2 cells from apoptosis induced as a consequence of serum-deprivation. T2 cells undergo a significant apoptotic response (involving >60% of the population) following 3 days in serum-free medium. Addition of the stable 14-1b recombinant PAI-1 protein (but not BSA), in final concentrations of 0.02 to 100 nM at the time of change-over to FBS-deficient medium, protected T2 cells from apoptosis due to serum-deprivation. (A) The significant (p < 0.005) apoptotic “rescue” evident in cultures incubated in 20 nM PAI-1 (B) correlated with a PAI-1-related increase in ERK1/2 phosphorylation (C) consistent with previous observations that PAI-1 initiates signaling events in various vascular and non-vascular cell types.30 Data plotted (in A, B) is the mean ± SD of triplicate independent experiments.