Table 1.
Addition of the stable PAI-1 mutant protein 14-1b inhibits serum deprivation-induced apoptosis in RK cells.
| Culture conditionsa | Percent apoptotic cellsb |
|---|---|
| FBS-free | 10.7 ± 1.2 |
| FBS-free + 20 nM PAI-1 | 3.3 ± 1.7 |
| FBS-free + 100 nM PAI-1 | 0.1 ± 0.1 |
a
RK cells were grown to 50% confluency, the conditioned medium removed, cells layers rinsed in PBS twice and serum-free DMEM ± PAI-1 14-1b (in the concentrations indicated) added for a 3-day incubation period.
b
Apoptotic cells were scored using DAPI to identify condensed chromatin at the nuclear periphery and fragmented nuclear bodies.