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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Artif DNA PNA XNA. 2010 JAN–MAR;1(2):1–7.

Figure 2. Detection discrimination of methicillin-sensitive (MSSA) versus methicillin-resistant (MRSA) strains.

Figure 2

Images of S. aureus bacterial cells observed by fluorescent microscopy in experiments performed with simulated matrix (packed red cells and blood culture media) spiked with S. aureus bacterial cells. A combination of two probes (SA-3 and MR-1) was applied to simulated matrix specimens spiked with: the regular MSSA strain (A1, B1); the Mu3 MRSA strain (A2, B2) and their mixture (A3, B3, C3). The fluorescent signals were acquired separately using three filter sets: A1, A2, and A3 present a superposition of two separate images, with DAPI and CY3; B1, B2, and B3 present a superposition of two separate images, with DAPI and FITC; C3 presents a superposition of three separate images, with DAPI, CY3 and FITC.