Fig. 4.

Deregulation of Ahr pathway genes in activated E2F2^−/− T lymphocytes. (A, C) Western analysis of Ahr and Arnt expression. Values were normalized against β-actin. The ratios Ahr/β-actin and Arnt/β-actin in WT were considered as a unit. The experiment shown is representative of three independent experiments (n = 8 WT; n = 8 E2F2^−/−). (B, D, E) Quantitative RT-PCR analysis of Ahr, Arnt and Ahrr. mRNA expression values were normalized against the mRNA expression levels of an irrelevant gene (eEf1a1) and given as relative values (n = 6 WT; n = 6 E2F2^−/−; two independent experiments, each in triplicate).