Table II. Properties of intrinsically disordered domains in S. cerevisiae FG nucleoporins.
MG, molten globule; PMG, premolten globule.
| Nucleoporin | Disordered domain AAsa | FG motifs | Net charge | pI | Charged AAs (DEKR) | Hydrophobic AAs (AILFWV) | Charged to hydrophobic ratio | Molecular mass | Configuration at 30 °C | Predicted Rh at 30 °Cb | Predicted diameter | Volume | Number per NPC | Volume × number | FG domain interactionc |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % | % | daltons | nm | nm | nm3 | nm3 | |||||||||
| Nup159 | 387–1071 | 16 | −38 | 4.7 | 24 | 22 | 1.1 | 71,748 | Relaxed coil | 6.7 | 13.4 | 1,259 | 8 | 10,074 | Repulsive |
| Nup42 | 1–382 | 20 | +11 | 10.7 | 4 | 25 | 0.2 | 37,577 | PMG | 4.1 | 8.2 | 289 | 8 | 2,309 | Cohesive |
| Nup116 | 172–764 | 42 | +11 | 10.7 | 2 | 22 | 0.1 | 59,006 | PMG | 4.9 | 9.8 | 492 | 8 | 3,940 | Cohesive |
| Nup116 | 765–960 | 0 | −1 | 6.3 | 35 | 26 | 1.4 | 22,152 | Relaxed coil | 3.8 | 7.6 | 230 | 8 | 1,838 | Repulsive |
| Nup100 | 1–610 | 43 | +12 | 11.4 | 2 | 21 | 0.1 | 61,153 | PMG | 4.9 | 9.8 | 493 | 8 | 3,941 | Cohesive |
| Nup100 | 611–800 | 0 | 0 | 7.2 | 26 | 25 | 1.0 | 20,842 | Relaxed coil | 3.7 | 7.4 | 212 | 8 | 1,697 | Repulsive |
| Nsp1 | 1–186 | 13 | +4 | 10.5 | 2 | 22 | 0.1 | 18,507 | PMG | 3.1 | 6.2 | 125 | 32 | 3,991 | Cohesive |
| Nsp1 | 187–617 | 22 | +4 | 8.7 | 36 | 24 | 1.5 | 44,749 | Extended coil | 6.2 | 12.4 | 998 | 32 | 31,930 | Repulsive |
| Nup49 | 1–251 | 17 | +7 | 10.7 | 3 | 24 | 0.1 | 24,320 | MG | 2.5 | 5.0 | 65 | 16 | 1,047 | Cohesive |
| Nup57 | 1–255 | 15 | +7 | 10.8 | 3 | 21 | 0.1 | 24,601 | PMG | 3.4 | 6.8 | 165 | 16 | 2,633 | Cohesive |
| Nup145N | 1–242 | 13 | +6 | 10.4 | 3 | 17 | 0.2 | 23,931 | MG | 2.5 | 5.0 | 65 | 16 | 1,047 | Cohesive |
| Nup145N | 243–433 | 1 | +2 | 8.8 | 24 | 27 | 0.9 | 20,930 | PMG | 3.2 | 6.4 | 137 | 16 | 2,195 | Repulsive |
| Nup1 | 220–797 | 19 | +12 | 9.3 | 26 | 24 | 1.1 | 61,634 | Extended coil | 6.8 | 13.6 | 1,316 | 8 | 10,531 | Repulsive |
| Nup1 | 798–1076 | 10 | +7 | 11.8 | 4 | 26 | 0.2 | 27,525 | PMG | 3.6 | 7.2 | 195 | 8 | 1,563 | Cohesive |
| Nup60 | 389–539 | 4 | −3 | 5.3 | 26 | 27 | 1.0 | 16,576 | Relaxed coil | 3.3 | 6.6 | 151 | 8 | 1,204 | Repulsive |
| Nup2 | 160–600 | 15 | −8 | 5.2 | 27 | 23 | 1.2 | 46,960 | Extended coil | 6.3 | 12.6 | 1,047 | 8 | 8,375 | Repulsive |
a Specific boundaries for the intrinsically disordered domains were defined in Denning and Rexach (22) according to a bimodal AA substitution rate in these nups during evolution. The boundaries for Nup1 and Nup2 were refined here according to the PONDR predictions shown in Fig. 1.
b The predicted Rh for each domain was derived from its molecular weight and its best match structural category using the scaling equations defined in Table I.
c These assignments are based on the interactions reported here or in Patel et al. (27); the assignments are consistent with the rules of cohesion discussed in the text.