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The subunit composition of complex I from P. pastoris. The subunits of complex I were separated by SDS-PAGE, using a 12-22% acrylamide gradient, and stained with Coomassie Blue R250. The bands were excised from the gel, digested with trypsin, and identified by peptide mass fingerprinting and tandem mass spectrometry. The subunits are labeled according to the SwissProt nomenclature.
