Categorization of uninjured and injured rats based on the percentage of mechanical threshold changes seen in the forelimbs after spinal cord injury (SCI), or sham treatment, versus pre-SCI baseline values, using K-means clustering. The y axis shows the number of rats analyzed, and the x axis the percentage change in forelimb mechanical thresholds at 4 weeks compared to baseline values (before sham treatment or SCI, set to 100%). (A) Sham rats (n = 25) showed a mean decrease of −0.23 ± 37.8%, while contused rats (SCI; n = 59) showed a mean decrease of −47 ± 34.93%. (B) After introducing a 40% criterion, a group of SCI rats (33 out of 59) showed significantly increased sensitivity to mechanical stimuli compared to sham rats.