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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: J Immunol Methods. 2009 Jan 31;343(1):28–41. doi: 10.1016/j.jim.2009.01.005

Fig. 4. BCR expression and antibody secretion in SP2ab derived hybridomas.

Fig. 4

A. Igα expression by hybridomas derived from the SP2/0 and SP2ab myeloma fusion partners. The samples are as follows: the two parental myeloma cell lines SP2/0 wild-type and SP2ab; hybridoma cell lines paired by their Ig isotypes derived from fusions with SP2/0 (IgM0, IgG10, IgG30) and SP2ab (IgMab, IgG1ab, IgG3ab). B. Expression of Igα in hybridomas producing the IgG2a isotype. Two hybridomas each derived from SP2/0 (IgG2a0) and SP2ab (IgG2aab) are compared. A & B. Moderate to high levels of cell surface expression (S.E.) of Ig by the various cell lines (Fig. 5) was scored as positive, negative (background levels). Details of protein loading and detection on these Western blots were described previously (Kandasamy et al., 1999) (also see Fig. 2). C. Secretion of Ab into growth media by hybridomas derived from the SP2/0 (n =15) and SP2ab (n =25) myeloma parents was quantified by ELISA. The standard deviations are shown.