Ingredient	Amount	Notes
BSA 5%	0.5 g	Fraction V
MEM liquid	94.0 ml	Sigma
DMEM liquid	80.0 ml	Sigma
F-12 liquid	20.0 ml	Sigma
Glucose 45% liquid	1.50 ml	Sigma solution
Glutamine 200mM	0.5 ml	Aliquotted Sigma solution
Diporzio Conc.	2.0 ml	Sigma solution
Liquid Catalase	0.1 ml
Kynurenic acid 0.5M	200μl	In 1N NaOH
HCL 5N	50 μl