NEED | Combine | Alliquot | ||||||
Additive | Solvent | Tube | Amount | ml | ml/tube | Conc. | Amount | # Aliq. |
Insulin | 20mM HCL (1) | plastic | 250mg bottle | 10 | 1 | 25mg/ml | 25mg | 10 |
Transferrin | Hank’s | 500mg bottle | 5 | 1 | 100mg/ml | 100mg | 5 | |
SOD | Hank’s | 70mg bottle | 14 | 1 | 5mg/ml | 5mg | 14 | |
Putrescine | Hank’s | 50mg | 3 | 0.12 | 20mg/ml | 2.4mg | 21 | |
Na2SeO3 | Hank’s | 0.104mg | 10 | 0.5 | 10μg/ml | 5.2μg | 20 | |
T3 | 10mM NaOH | 2mg | 10 | 0.1 | 0.20mg/ml | 0mg | 100 | |
Progesterone | 100% EtOH | Glass | 12.5mg | 10 | 0.05 | 1.25mg/ml | Use pipet | |
Cortisol | 100% EtOH | Glass | 20mg | 10 | 0.02 | 2.00mg/ ml | Use pipet |