Ingredient | Amount | (Notes) Final Conc. |
Cysteine Water | 7.8mL | 1mM cysteine |
Papain | Varies (*190μl for the bottle of 44.0mg/ml) | 20 units/ml (400 units total for 20ml worth of sol’n) |
H&B conc. | 2mL | |
Carbogen | 95% O2 + 5% CO2 | |
HCl 5N | 10μl | |
0.5% Phenol red | 20μl | 0.001% |
Kynurenate 0.5M | 10μl | (In 1N NaOH) 0.5mM |