| Ingredients | M.W. | Powder/50ml H2O | Conc. (M) | Combine (ml) | Final Conc. (mM) |
| NaCl | 58.44 | 11.699 g | 4 | 14.5 | 116 |
| KCl | 74.56 | 3.728 g | 1 | 2.7 | 5.4 |
| NaHCO3 | 84.01 | 4.2 g | 1 | 13 | 26 |
| NaH2PO4*H2O | 137.99 | 6.90 g | 1 | 1 | 2 |
| MgSO4 | 120.38 | 6.019 g | 1 | 0.5 | 1 |
| EDTA (ED2- SS) | 292 | Sigma 5% (make 5g/100ml stock) | 0.134 | 0.3722 | 0.5 |
| Glucose | 180 | Sigma 45% | 2.5 | 5 | 25 |
| TC H2O | 62.93 |
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