Skip to main content
. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Apr 23;396(2):272–277. doi: 10.1016/j.bbrc.2010.04.077

Fig. 4.

Fig. 4

Poly I:C and R-848 elevate GPR33 mRNA expression in vivo. C57BL/6 mice at the age of 9 weeks were treated intraperitoneal and alternatively nasal with A: poly I:C and B: R-848 or 0.9#NaCl solution as control. After 9 h mice were sacrificed and total RNA was isolated from lung and the major lymphoid tissues. The mRNA levels of the GPR33 transcripts were quantified by qPCR assays. Data are presented as GPR33 mRNA expression x-fold over negative control (organs from the 0.9% NaCl solution group) as mean ± SEM of n ≥ 4 independent experiments performed in duplicates. Control ΔCts: spleen (i.p. 8.39±0.16, nasal 8.87±0.23), lymph nodes (i.p. 8.22±0.25, nasal 8.55±0.15), lung (i.p. 9.70±0.26, nasal 9.62±0.11) and thymus (i.p. 8.50± 0.19, nasal 9.22±0.12). *p < 0.05; **p < 0.01; ***p < 0.001.