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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Aging Cell. 2008 Sep 8;7(6):824–835. doi: 10.1111/j.1474-9726.2008.00434.x

Figure 1. Oligomeric Aβ, but not monomeric or fibrillar Aβ, induces cell death SY5Y cells.

Figure 1

(A) Left: Representative dot blot analysis detecting monomeric, oligomeric and fibrillar human Aβ1–42 from standard preparations spotted onto nitrocellulose membranes. Membranes were probed with the oligomer-specific A-11 antibody (top) or the 6E10 antibody (which recognizes Aβ sequence, regardless of conformation). Right: representative western blot analysis employing the A-11 antibody to detect oligomers in a typical preparation of oligomeric Aβ1–42. Dimers, trimers and dodecamers are shown to be the most prevalent oligomeric species.

(B) LDH release cytotoxocity assay in human SY5Y neuroblastoma cells treated for 12 hr with increasing concentrations of monomerc, oligomeric or fibrillar Aβ. Dashed line represents LDH release value in control cells. Values are mean ± SD of 4 independent replicates. Experiment was repeated twice with similar results. *: p<0.001 vs. control cells (ANOVA).