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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Aging Cell. 2008 Sep 8;7(6):824–835. doi: 10.1111/j.1474-9726.2008.00434.x

Figure 6. Oligomeric Aβ, but not monomeric or fibrillar Aβ, decreases CREB-driven transcriptional activity in SY5Y cells in a CaN-dependent fashion.

Figure 6

(A) SEAP activity assay in the culture medium of SY5Y cells transiently transfected with CREB-sensitive pCRE-SEAP or the CREB-insensitive pTAL-SEAP reporter gene and treated for 3 hr with 0.75 μM monomeric, oligomeric or fibrillar Aβ in the presence or absence of the adenilate cyclase activator forskolin (10 μM). N=4 independent replicates per group; *: p<0.01 vs. forskolin-treated vehicle cells (ANOVA).

(B) SEAP activity assay in the culture medium of SY5Y cells transiently transfected with pCRE-SEAP reporter gene and treated for 3 hr with 0.75 μM oligomeric Aβ in the presence of 10 μM forskolin (FRSK). Parallel cells were additionally treated with 1 or 5 μM FK506. N=3–4 independent replicates per group; *: p<0.05 vs. forskolin+oligomeric Aβ-treated cells (ANOVA).