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. 2010 Oct 15;21(20):3506–3517. doi: 10.1091/mbc.E10-03-0259

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 10. — siRNA-mediated depletion of α-adducin accelerates TPA-induced AJ disassembly. (A) Immunoblots of cell lysates collected from control and α- or γ-adducin siRNA-transfected HPAF-II pancreatic cell monolayers demonstrate a dramatic decrease in adducins expression on day 4 after transfection. *p < 0.05 compared with control siRNA-transfected cells (n = 3). (B) Control and α- adducin–depleted HPAF-II cells were exposed to TPA for 3 h. Immunofluorescence labeling and confocal microscopy show phorbol ester-induced AJ fragmentation and internalization of E-cadherin-containing vesicles (arrows) in control cell monolayer. Such AJ disintegration is significantly exaggerated in α-adducin–depleted HPAF-II cells (arrowheads). Bar, 10 μm.